Hannah Weston, 26, said it took her several days to complete the 4ft by 3ft (1.2m by 0.9m) homage to the boy wizard.
But the painting never made it to the Parcelforce depot near her Norwich home.
The firm said it was "unable to locate" it and offered £125 in compensation.
Tattoo artist Ms Weston, 26, said the situation was "incredibly disheartening".
"I poured days of my time, energy and passion into that huge painting," Ms Weston told the BBC.
"I painted Harry out of pure adoration for stories that have brought me joy and I hope that it ends up in the hands of someone who truly appreciates it."
She sold the painting to a woman who planned to give it to her daughter, who was "obsessed with the Harry Potter books", as a birthday present.
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